1 Division of DNA Repair and Genome Stability, Department of Radiation Oncology, Dana-Farber/Brigham and Women's Cancer Center, Harvard Medical School, Boston, MA, USA 2 2Division of Medical Physics ...

For decades, optimizing annealing temperatures in PCR has pushed the limits of PCR forward to more difficult tasks. At the same time, mastermixes and kits became more powerful and less prone for ...

Anyone who has worked with DNA in the lab is probably (all too) familiar with a very special reaction, the polymerase chain reaction, or PCR. The technique allows one to make an enormous number of ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

Failed polymerase chain reactions (PCRs) are a common cause of frustration in the lab. While a typical PCR protocol is quite straightforward, scientists must optimize each step to amplify the intended ...

PCR, or Polymerase Chain Reaction, is a revolutionary molecular biology technique that allows for the rapid amplification of specific DNA sequences. It is an essential tool in various fields, ...

Taq polymerase is the heat-stable (thermostable) DNA polymerase extracted from the thermophilic bacteria Thermus aquaticus. Its predominant function is in the polymerase chain reaction (PCR) technique ...

To alleviate the limitations discussed above, we developed a novel PCR-based application – coamplification at lower denaturation temperature (COLD)-PCR, [24] that preferentially enriches minority ...